Advances in two-dimensional electrophoresis

Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE): advances and perspectives.

The recent trend in science is to assay as many biological molecules as possible within a single experiment. This trend is evident in proteomics where the aim is to characterize thousands of proteins within cells, tissues, and organisms. While advances in mass spectrometry have been critical, developments made in two-dimensional PAGE (2D-PAGE) have also played a major role in enabling proteomic...

Two-dimensional Electrophoresis

Two-dimensional (2D) polyacrylamide gel electrophoresis is a classical technique for the separation of proteins. It Rrst appeared in the mid-1970s but for a long time it only found limited applications. Recently it has enjoyed an impressive renaissance. The major reasons for this are the introduction of the immobilized pH gradient (IPG) strips and the development of analytical methods capable o...

Two-Dimensional Electrophoresis

We compared cellular proteins from normal human blood lymphocytes, monocytes, and granulocytes, using highresolution two-dimensional electrophoresis. The leukocytes were isolated from peripheral blood by centrifugation on density step gradients (yielding a fraction of purified granulocytes and a lymphocyte/monocyte mixture) and monocytes were subsequently separated from lymphocytes by virtue of...

Two-dimensional Gel Electrophoresis

Two Dimensional Gel Electrophoresis in Cancer Proteomics

Two-dimensional electrophoresis (2-DE) is a powerful and widely used method for the analysis of complex protein mixtures extracted from cells, tissues, or other biological samples. This technique sort’s protein according to two independent properties in two discrete steps: the first-dimension step, isoelectric focusing (IEF), separates proteins according to their isoelectric points (pI); the se...