Advances in targeted genome editing

Advances in Targeted Drug Delivery

Genome Editing With Targeted Deaminases

Luhan Yang, Adrian W. Briggs*, Wei Leong Chew*, Prashant Mali, Marc Guell, John Aach, Daniel Bryan Goodman, David Cox, Yinan Kan, Emal Lesha Venkataramanan Soundararajan, Feng Zhang, George Church 1 Department of Genetics, Harvard Medical School, Boston, MA 2 Program in Biological and Biomedical Sciences, Harvard Medical School, Boston, MA 3 Harvard-MIT Division of Health Science and Technology...

Advances in Targeted Drug Delivery

Advances in Nucleases Used for Genome Editing

Genome editing is an exciting technology that allows for specific manipulation of complex genomes. While the original tools for genome manipulation had low efficiencies, genome editing tools discovered in the past fifteen years have been widely studied and great efforts have been required to improve their efficiency. This article summarizes how Zinc Finger Nuclease (ZFN), Transcription Activato...

Recent Advances in Genome Editing Using CRISPR/Cas9

The CRISPR (clustered regularly interspaced short palindromic repeat)-Cas9 (CRISPR-associated nuclease 9) system is a versatile tool for genome engineering that uses a guide RNA (gRNA) to target Cas9 to a specific sequence. This simple RNA-guided genome-editing technology has become a revolutionary tool in biology and has many innovative applications in different fields. In this review, we brie...