ADP-ribosylation in isolated nuclei of Physarum polycephalum
نویسندگان
چکیده
منابع مشابه
ADP-ribosyltransferase in isolated nuclei during the cell cycle of Physarum polycephalum.
ADP-ribosyltransferase was measured in isolated nuclei of Physarum polycephalum. Activity was determined with and without exogenous DNA and histones. During the synchronous cell cycle the activity measured with exogenous substrates exhibited a typical peak enzyme pattern with a maximum of activity in S-phase, whereas activity measured without exogenous substrates displayed a step enzyme pattern...
متن کاملPoly(adenosine diphosphate ribose) polymerase in Physarum polycephalum nuclei.
1. The isolated nuclei of the slime mould Physarum polycephalum contain an enzyme that will incorporated [adenine-3H] NAD+ into an acid-insoluble product, which is shown to be poly(ADP-ribose). 2. This incorporation has an optimum pH of 8.2 and a temperature optimum below 10degreesC. 3. Optimum stimulation is given by 15 mM-Mg2+. 4. 2-Mercaptoethanol or dithiothreitol also stimulates the incorp...
متن کاملViability of Physarum polycephalum spores and ploidy of plasmodial nuclei.
Amoebae of Physarum polycephalum carrying the mth mating-type allele may differentiate into plasmodia in the absence of mating. Such plasmodia are haploid and, upon sporulation, produce mainly inviable spores. We have asked whether the viable spores arise from meiotic or mitotic divisions. Using a microfluorometric measurement of the deoxyribonucleic acid content of individual nuclei, we found ...
متن کاملLogical Modelling of Physarum Polycephalum
In the paper we proposed a novel model of unconventional computing where a structural part of computation is presented by dynamics of Plasmodium of Physarum polycephalum, a large single cell. We sketch a new logical approach combining conventional logic with process calculus to demonstrate how to employ formal methods in design of unconventional computing media presented by Physarum polycephalu...
متن کاملPhysarum polycephalum Histones
Physarumpolycephalum is an acellular slime mould whose histones are of general interest because they allow comparisons to be made between lower eukaryote and higher eukaryote histones and, perhaps more importantly, they provide a unique opportunity for the study of histone changes in the mitotic cycle, which is naturally synchronous in P. polycephalum plasmodia, and during differentiation. Stud...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1988
ISSN: 0264-6021,1470-8728
DOI: 10.1042/bj2530859