AdipoR2 inhibits human glioblastoma cell growth through the AMPK/mTOR pathway

Abstract Background AdipoR2, which belongs to the seven-transmembrane-domain receptor family, has been shown play an important role in development of human tumours, but underlying mechanisms are poorly understood. In this study, we found that AdipoR2 expression correlates with glioma grade. addition, also investigated behind antiproliferative effects U251 cells (a cell line) using colony formation and WST-8 growth assays. Methods The line was cultured vitro. Western blotting used detect relevant proteins. Quantitative RT-PCR AdipoR1 expression. Flow cytometry cycle assay results. gene profiles samples from CGGA database were analysed by MATLAB GSEA software. Results AMPK/mTOR pathway plays a central regulation proliferation, differentiation migration may promote tumorigenesis. Therefore, can control cancer progression modulating pathway. However, there is no information on relationship between AdipoR nervous system tumours such as GBM. study. We 648 upregulated genes 436 downregulated correlated 158 samples. suggested cycle-associated gene. results flow analysis indicated induced G0/G1 arrest cells. Furthermore, identified signalling axis be involved AdipoR2-induced arrest. Conclusions Our suggest represent novel endogenous negative regulator GBM proliferation. These findings promising therapeutic target patients.