Adenosine metabolism in phytohemagglutinin-stimulated human lymphocytes.
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چکیده
منابع مشابه
Adenosine metabolism in phytohemagglutinin-stimulated human lymphocytes.
The association of a human genetic deficiency of adenosine deaminase activity with combined immunodeficiency prompted a study of the effects of adenosine and of inhibition of adenosine deaminase activity on human lymphocyte transformation and a detailed study of adenosine metabolism throughout phytohemagglutinin-induced blastogenesis. The adenosine deaminase inhibitor, coformycin, at a concentr...
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Poliovirus replication has been studied in human lymphocytes during the course of blastogenesis under phytohemagglutinin (PHA) stimulation. Enhancement of virus replication in PHA-stimulated leukocyte cultures was due to an increase in number of virus-producing cells. Virus yield was approximately 10 plaque-forming units (PFU) per producing cell, both in stimulated and in nonstimulated cultures...
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I T HAS BEEN SHOWN that peripheral i)lood lynlphocvtes undergo trainsformation and mitosis in vitro when cultured in the presence of plwtohemagglutinin ( PHA).’4 Little information has been reported on the numl)er of lymphocytes which survive and transform during the culturing interval. It has been very difficult to enumerate lymphocytes in cultures containing PFIA I)ecause the agglutinated ery...
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W HEN HUMAN LYMPHOCYTES are treated with phytohemagglutinin ( PHA ) , an extract of the kidney bean Phaseolus vulgaris, they undergo morphologic transformation followed by mitosis.13 Cooper and Rubin4 have demonstrated a decrease in the amount of total RNA witin 30 minutes after the exposure of lymphocytes to PHA. This is immediately followed by an exponential increase in the synthesis of RNA w...
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ژورنال
عنوان ژورنال: Journal of Clinical Investigation
سال: 1976
ISSN: 0021-9738
DOI: 10.1172/jci108512