Adenosine 5′-sulphatophosphate kinase activity in spinach leaf tissue
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چکیده
منابع مشابه
Assay of adenosine 3'-phosphate 5'-sulphatophosphate in hepatic tissues.
A fluorimetric assay, based on the ability of boiled hepatic extracts to support the sulphO-conjugation of harmol, was used to demonstrate and quantify PAdoPS (adenosine 3'-phosphate 5'-sulphatophosphate) present in liver. A stoicheiometric relationship was established between the sulphate conjugate formed and the 'active sulphate' utilized. Guinea-pig, rat, mouse and rabbit livers contain 3.3,...
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The current methods for the determination of creatine kinase (EC 2.7.3.2) activity are derived from Oliver's method, in which AMP is used to decrease interference by adenylate kinase (EC 2.7.4.3). Recently, Szasz et al. and Rosano et al. described methods in which diadenosine pentaphosphate and fluoride, respectively, are used to reduce this interference. However, diadenosine pentaphosphate doe...
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suggesting the rapid formation of one or more complexes (C) between the two proteins within which electron transfer takes place. Attempts to detect directly the presence of a thermodynamically stable complex between the various forms of the two proteins are presently being carried out. An explanation of the mechanism of electron transfer between azurin and cytochrome 551, in analogy with lines ...
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The effects of epinephrine, glucagon, insulin and 1-methyl-3-isobutylxanthine on adenosine 3:5-monophosphate (cAMP)-dependent protein kinase activity were investigated in the perfused rat heart. The conditions for homogenization of heart tissue and assay of protein kinase are described. The activation state of the enzyme is expressed as the ratio of the rate of phosphorylation of histone in the...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1973
ISSN: 0306-3283
DOI: 10.1042/bj1340565