Activity Levels of Amine Transaminases Correlate with Active Site Hydrophobicity

Studies on the active site of pig plasma amine oxidase.

Amine oxidase from pig plasma (PPAO) has two bound Cu2+ ions and at least one pyrroloquinoline quinone (PQQ) moiety as cofactors. It is shown that recovery of activity by copper-depleted PPAO is linear with respect to added Cu2+ ions. Recovery of e.s.r. and optical spectral characteristics of active-site copper parallel the recovery of catalytic activity. These results are consistent with both ...

PROBING THE ACTIVE SITE OF RHODANESE WITH DISULFIDE REAGENTS

Identification, characterization and application of novel (R)-selective amine transaminases

Modern tools for enzyme discovery andprotein engineering substantially broadened thenumber of enzymes applicable for biocatalysis andhelped to alter their properties such as substraterange, enantioselectivity, and stability under processconditions. In addition, these methods also enabledone to explore reactions for organic synthesis forwhich no suitable enzymes were ...

Serum Renalase Levels Correlate with Disease Activity in Lupus Nephritis

INTRODUCTION Lupus nephritis (LN) is among the most serious complications of systemic lupus erythematosus (SLE), which causes significant morbidity and mortality. Renalase is a novel, kidney-secreted cytokine-like protein that promotes cell survival. Here, we aimed to investigate the relationship of serum renalase levels with LN and its role in the disease progression of LN. METHODS For this ...

Amine Transaminases in Multi-Step One-Pot Reactions

Amine transaminases are enzymes that catalyze the mild and selective formation of primary amines, which are useful building blocks for biologically active compounds and natural products. In order to make the production of these kinds of compounds more efficient from both a practical and an environmental point of view, amine transaminases were incorporated into multi-step one-pot reactions. With...