ACTIVITIES OF L-ASPARAGINASE FROM CICER COTYLEDONS UNDER DIFFERENT TREATMENTS

Specific activities and transcript levels of glycolytic enzymes under dehydration in chickpea (Cicer arietinum L.) seedlings

The effect of dehydration on the specific activities and transcript levels of several glycolytic enzymes was determined in two chickpea (Cicer arietinum L.) cultivarsPusa362 (drought tolerant) and SBD377 (drought sensitive). Dehydration treatment was imposed on 6 days old chickpea seedlings by removing them from pots and keeping them on table top on 3MM Whatman paper at room temperature for 5 h...

Preformed mRNA in Cotyledons of Ungerminated Seeds of Cicer arietinum L.

Polyadenylated RNA was isolated from total RNA extracted from cotyledons of ungerminated or 18-hour-germinated chick-pea seeds by affinity chromatography on oligo(dT)-cellulose. Both poly(A)-containing RNA fractions exhibited a template activity when assayed in two cell-free translation systems, wheat germ extracts, and nuclease-treated reticulocyte lysates. Translation of preformed mRNA from c...

Comparative Study of L-asparaginase Activity in Different Bacterial Species

Accumulation of Phenolic Compounds and Phytoalexins in Sliced and Elicitor-Treated Cotyledons of Cicer arietinum L

Cicer arietinum, Ascochyta rabiei, Elicitor. Isoflavones, Phytoalexins Upon slicing cotyledons of chickpea, Cicer arietinum L ., accumulate the pterocarpan phyto­ alexins medicarpin and maackiain. Treatment of this tissue with an elicitor from the phytopathogenic deuteromycete Ascochyta rabiei (Pass.) Lab. greatly enhances accumulation of the pterocarpans and of other isoflavones and flavonoids...

L-Asparaginase from Erwinia carotovora

The physicochemical properties of the antileukemic Lasparaginase from Erwinia carotovora have been studied and compared with those of the corresponding clinically effective enzyme from Escherichia coli B. Although both enzymes are tetramers with similar molecular weight, the amino acid compositions are distinctly different and the Erwinia enzyme is more basic as judged by polyacrylamide electro...