منابع مشابه
Escherichia coli rep helicase unwinds DNA by an active mechanism.
DNA helicases unwind duplex DNA to form the single-stranded (ss) DNA intermediates required for replication, recombination, and repair in reactions that require nucleoside 5'-triphosphate hydrolysis. Helicases generally require a ss-DNA flanking the duplex in order to initiate unwinding in vitro; however, the precise function of the ss-DNA is not understood. If a helicase unwinds DNA by a "pass...
متن کاملDNA-induced dimerization of the Escherichia coli Rep helicase.
The Escherichia coli Rep protein is a DNA helicase that is involved in DNA replication. We have examined the effects of DNA binding on the assembly state of the Rep protein using small-zone gel permeation chromatography and chemical crosslinking of the protein. Complexes of Rep protein were formed with short single-stranded and duplex hairpin oligodeoxynucleotides with lengths such that only a ...
متن کاملAutoinhibition of Escherichia coli Rep monomer helicase activity by its 2B subdomain.
DNA helicases catalyze separation of double-helical DNA into its complementary single strands, a process essential for DNA replication, recombination, and repair. The Escherichia coli Rep protein, a superfamily 1 DNA helicase, functions in DNA replication restart and is required for replication of several bacteriophages. Monomers of Rep do not display helicase activity in vitro; in fact, DNA un...
متن کاملAuto-inhibition of E. coli Rep Monomer Helicase Activity by its 2B Sub-domain
2 DNA helicases catalyze separation of double helical DNA into its complementary single strands, a process essential for DNA replication, recombination, and repair. The E. coli Rep protein, a superfamily 1 (SF1) DNA helicase, functions in DNA replication restart and is required for replication of several bacteriophages. Monomers of Rep do not display helicase activity in vitro; in fact, DNA unw...
متن کاملUvrD helicase, unlike Rep helicase, dismantles RecA nucleoprotein filaments in Escherichia coli.
The roles of UvrD and Rep DNA helicases of Escherichia coli are not yet fully understood. In particular, the reason for rep uvrD double mutant lethality remains obscure. We reported earlier that mutations in recF, recO or recR genes suppress the lethality of uvrD rep, and proposed that an essential activity common to UvrD and Rep is either to participate in the removal of toxic recombination in...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2020
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2019.11.2147