ACTIONS OF ION-TRANSPORT PEPTIDE FROM LOCUST CORPUS CARDIACUM ON SEVERAL HINDGUT TRANSPORT PROCESSES
نویسندگان
چکیده
منابع مشابه
Actions of Ion-transport Peptide from Locust Corpus Cardiacum on Several Htndgut Transport Processes
1. Schistocerca gregaria ion-transport peptide (Scg-ITP), a neuropeptide isolated from locust corpora cardiaca, stimulates ileal Cl~ transport (/«:) in a dose-dependent manner and causes increases in Na, K (/K) and fluid reabsorption (7V) as previously observed with crude extracts of corpus cardiacum and with cyclic AMP. Unlike cyclic AMP, ScgITP does not stimulate ileal NH4 secretion. 2. H sec...
متن کاملIsolation of a neuropeptide from locust corpus cardiacum which influences ileal transport.
1. Schistocerca gregaria ion-transport peptide (Scg-ITP) was isolated from aqueous extracts of the corpus cardiacum by a four-step procedure, utilizing reverse-phase high-performance liquid chromatography for separation and stimulation of a Cl(-)-dependent short-circuit current (Isc) across locust ilea as the bioassay. 2. Scg-ITP has an unblocked N terminus and an apparent relative molecular ma...
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Ion transport peptide (ITP) purified from locust nervous corpus cardiacum (CC) has previously been shown to stimulate salt and water reabsorption and inhibit acid secretion in the ileum of Schistocerca gregaria. We used the partial amino acid sequence of purified ITP to derive degenerate primers. These were used to amplify a cDNA from brain RNA using reverse transcription and the polymerase cha...
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1. The commonly used method of passing short-circuit current (/sc) across insect epithelia through Ag-AgCl electrodes, without the use of salt bridges, leads to significant OH~ production at the cathode (lumen side) when high currents are applied. 2. The alkalization of the lumen previously reported when cyclic AMP was added to short-circuited locust hindgut is a result of this phenomenon rathe...
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ژورنال
عنوان ژورنال: Journal of Experimental Biology
سال: 1992
ISSN: 1477-9145,0022-0949
DOI: 10.1242/jeb.173.1.275