Actions of FGF2, LIF, and IGF1 on bovine embryo survival and conceptus elongation following slow-rate freezing

Culture environment during in vitro embryo production can affect phenotype and pregnancy outcomes, making culture modifications a logical approach for improving competence. Previously, the addition of growth factors FGF2, LIF, IGF1, termed FLI, to medium improved bovine development, re-expansion following cryopreservation. The objective this study was investigate survival cryopreserved FLI treated embryos at day 15 evaluate conceptus transcriptomes. Embryos were produced using fertilization abattoir-derived oocytes, cultured blastocyst stage presence or absence (+/- FLI), by slow-rate freezing. Thawed transferred into non-lactating recipient beef cows eight days later conceptuses recovered analyzed. For subset whole transcriptome analysis performed NovaSeq 6000. There no detectable difference recovery average length between two groups. 32 differentially expressed transcripts, 23 up-regulated nine down-regulated +FLI group compared -FLI. Genes involved interferon signaling, prostaglandin synthesis, placental development. This reveals that with without freezing have similar developmental competence up Nevertheless, differences gene expression exhibit an effect on signaling elongation.