Actinomycin Synthesis in Washed Cells of Streptomyces antibioticus
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چکیده
منابع مشابه
The possible involvement of a plasmid(s) in actinomycin synthesis by Streptomyces parvulus and Streptomyces antibioticus.
The treatment of Streptomyces parvulus and Streptomyces antibioticus with acriflavine or novobiocin resulted in the loss of ability to produce actinomycin. The concomitant loss of ability to form aerial mycelium and the incidence of auxotrophic progeny (S. parvulus) were both low relative to the loss of the antibiotic-producing property. Protoplast fusion induced by polyethylene glycol 4000, us...
متن کاملMorphological changes accompanying actinomycin production in Streptomyces antibioticus.
The fine structure of the actinomycin producing bacterium Streptomyces antibioticus has been studied. The typical appearance of freshly ineculated cells is one of a generally electron dense cytoplasm. As cellular protein synthesis ceases and secondary metabolism (actinomycin biosynthesis) is initiated, a gradual loss of cytoplasmic density occurs, revealing in many cells membrane enclosed vesic...
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Using insertional mutagenesis, we have disrupted the RNase III gene, rnc, of the actinomycin-producing streptomycete, Streptomyces antibioticus. Disruption was verified by Southern blotting. The resulting strain grows more vigorously than its parent on actinomycin production medium but produces significantly lower levels of actinomycin. Complementation of the rnc disruption with the wild-type r...
متن کاملPurification of RNA polymerase from actinomycin producing and nonproducing cells of Streptomyces antibioticus.
DNA-dependent RNA polymerase has been purified approximately 700-fold from 12-h-old cells of Streptomyces antibioticus and 400-fold from 48-h cells. Both enzymes appear nearly homogeneous as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Both enzymes possess subunits corresponding to the p, p’, and (Y subunits of Escherichia coli RNA polymerase but no band corresponding to...
متن کاملGenetic interrelations in the actinomycin biosynthetic gene clusters of Streptomyces antibioticus IMRU 3720 and Streptomyces chrysomallus ATCC11523, producers of actinomycin X and actinomycin C
Sequencing the actinomycin (acm) biosynthetic gene cluster of Streptomyces antibioticus IMRU 3720, which produces actinomycin X (Acm X), revealed 20 genes organized into a highly similar framework as in the bi-armed acm C biosynthetic gene cluster of Streptomyces chrysomallus but without an attached additional extra arm of orthologues as in the latter. Curiously, the extra arm of the S. chrysom...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1965
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)97072-9