Actin-Activated Atpase and Actin Sliding Velocities are Similarly Influenced by Actin-Myosin Binding Kinetics
نویسندگان
چکیده
منابع مشابه
Regulation of the actin-activated ATPase activity of Acanthamoeba myosin I by cross-linking actin filaments.
The actin-activated Mg2+-ATPase activity of phosphorylated Acanthamoeba myosin I was previously shown to be cooperatively dependent on the myosin concentration (Albanesi, J. P., Fujisaki, H., and Korn, E. D. (1985) J. Biol. Chem. 260, 11174-11179). This observation was rationalized by assuming that myosin I contains a high-affinity and a low-affinity F-actin-binding site and that binding at the...
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Based on the Stochastic Inclined Rods Model (SIRM) proposed by H. Matsuura and M. Nakano, we study the microscopic motion of actin myosin system including the motion of the G-actin. Our model is composed of an inclined spring (rod), a myosin head, a myosin filament and G-actins. We discuss the stochastic resonance between the myosin head and random noise. The results of calculation show that th...
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The studies of Szent-Gyorgyi (1) have clearly established the formation of actomyosin from F-actin and myosin A. SzentGyorgyi (l), Portzehl, Schramm, and Weber (2), Jaisle (3), Snellman and Gelotte (4), and Spicer and Gergely (5) have suggested from their viscometric and centrifugal studies that the maximal amount of myosin A which can be bound by F-actin is in the ratio of 2 to 4 : 1, by weigh...
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Myosin was isolated from the main pulmonary artery of swine and was phosphorylated or dephosphorylated by utilizing the endogenous kinase or phosphatase, respectively. The myosins, phosphorylated to various degrees, were purified free of kinase and phosphatase activities by gel filtration on Sepharose CL-4B agarose columns. The level of actin-activated ATPase activity was dependent upon the deg...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2014
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2013.11.4009