منابع مشابه
Structural basis of multiple drug-binding capacity of the AcrB multidrug efflux pump.
Multidrug efflux pumps cause serious problems in cancer chemotherapy and treatment of bacterial infections. Yet high-resolution structures of ligand transporter complexes have previously been unavailable. We obtained x-ray crystallographic structures of the trimeric AcrB pump from Escherichia coli with four structurally diverse ligands. The structures show that three molecules of ligands bind s...
متن کاملStructures of Gate Loop Variants of the AcrB Drug Efflux Pump Bound by Erythromycin Substrate
Gram-negative bacteria such as E. coli use tripartite efflux pumps such as AcrAB-TolC to expel antibiotics and noxious compounds. A key feature of the inner membrane transporter component, AcrB, is a short stretch of residues known as the gate/switch loop that divides the proximal and distal substrate binding pockets. Amino acid substitutions of the gate loop are known to decrease antibiotic re...
متن کاملPurification, crystallization and preliminary diffraction studies of AcrB, an inner-membrane multi-drug efflux protein.
Resistance of pathogens to antibiotics is often dependent on multi-drug export proteins that reside in the inner membrane of bacteria. This work describes the expression, purification, crystallization and preliminary crystallographic analysis of AcrB of Escherichia coli. Together with AcrA and TolC, AcrB forms a proton motive force dependent efflux pump of the resistance-nodulation-cell divisio...
متن کاملAssembly and Channel Opening in a Bacterial Drug Efflux Machine
Drugs and certain proteins are transported across the membranes of Gram-negative bacteria by energy-activated pumps. The outer membrane component of these pumps is a channel that opens from a sealed resting state during the transport process. We describe two crystal structures of the Escherichia coli outer membrane protein TolC in its partially open state. Opening is accompanied by the exposure...
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ژورنال
عنوان ژورنال: Annals of the New York Academy of Sciences
سال: 2019
ISSN: 0077-8923,1749-6632
DOI: 10.1111/nyas.14239