منابع مشابه
Characterization of a unique class C acid phosphatase from Clostridium perfringens.
Clostridium perfringens is a gram-positive anaerobe and a pathogen of medical importance. The detection of acid phosphatase activity is a powerful diagnostic indicator of the presence of C. perfringens among anaerobic isolates; however, characterization of the enzyme has not previously been reported. Provided here are details of the characterization of a soluble recombinant form of this cell-as...
متن کاملLocalized Secretion of Acid Phosphatase
Secretion of cell wall-bound acid phosphatase by Saccharomyces cerevisiae occurs along a restricted portion of the cell surface. Acid phosphatase activity produced during derepressed synthesis on a phosphate-limited growth medium is detected with an enzymespecific stain and is localized initially to the bud portion of a dividing cell . After two to three generations of phosphate-limited growth,...
متن کاملSoybean root nodule acid phosphatase.
Acid phosphatases are ubiquitous enzymes that exhibit activity against a variety of substrates in vitro, although little is known about their intracellular function. In this study, we report the isolation, characterization, and partial sequence of the major acid phosphatase from soybean (Glycine max L.) root nodules. The phosphatase was purified predominantly as a heterodimer with subunits of 2...
متن کاملAutomated determination of acid phosphatase.
A procedureis presentedfor the automated determination of acid phosphatase activity in biological materials from either plant or animal origin. The Technicon N-i flow diagram has been simplified, and the reagents for the measurement of enzymatically produced phenolic substanceshave been modified without lossof range or sensitivity. Both phenyiphosphateand a-naphthylphosphate, introduced by Babs...
متن کاملKinetic Properties of Acid Phosphatase
Introduction Phosphatases are enzymes that remove phosphate groups from substrates. Phosphorylated compounds are widely distributed in living systems. They serve as storage forms for energy (e.g., ATP and phosphocreatine), as components of informational macromolecules (i.e., nucleotides and deoxynucleotides), as allosteric effectors of certain enzymes (e.g., fructose-1-6bisphosphate), and as se...
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ژورنال
عنوان ژورنال: Japanese Journal of Microbiology
سال: 1970
ISSN: 0021-5139
DOI: 10.1111/j.1348-0421.1970.tb00504.x