Acetyltransferase P300 Inhibits the Proliferation, Invasion, and Migration of Esophageal Cancer via Survivin Acetylation

نویسندگان

چکیده

Background: Esophageal cancer is one of the primary death causes leading by in world, which high morbidity and mortality. Epigenetic acetylation modification participates regulates proliferation, invasion, metastasis various tumor cells, proteins involved acetyltransferases may be important mechanisms esophageal carcinogenesis. The aim this study was to investigate correlation acetyltransferase P300 Survivin pathogenesis its molecular mechanism. Methods: Fifty-five cases tissues adjacent were collected, protein expression measured immunohistochemistry (SP) blotting (Western Blot); acetylated levels coimmunoprecipitation (Co-IP); bioinformatics predicted relationship between as substrate, fluorescence (IF) verify localization tissues; acetylation, clinical characteristics analyzed statistics. siRNA sequences structured transfected into EC109 cells. determined Co-IP. Cell viability MTT assay, Scratch healing Transwell chamber assay examined cell migration invasion ability. Results: significantly increased human rate increased; that could catalyze a confirmed both simultaneously showed state expression; closely related with stage, tissue differentiation lymph node metastasis. vitro experiments RNA interference cells can reduce level, while inhibiting survival, capacity Conclusion: has lespathological process cancer, an upstream molecule potential value diagnosis treatment cancer.

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ژورنال

عنوان ژورنال: Chinese Medicine

سال: 2022

ISSN: ['2151-1926', '2151-1918']

DOI: https://doi.org/10.4236/cm.2022.134005