Acetone Bio-Sniffer (Gas-Phase Biosensor) for Monitoring of Human Volatile Using Enzymatic Reaction of Secondary Alcohol Dehydrogenase

We developed a highly sensitive acetone bio-sniffer (gas-phase biosensor) based on an enzyme reductive reaction to monitor breath concentration. The device was constructed by attaching flow-cell with nicotinamide adenine dinucleotide (NADH)-dependent secondary alcohol dehydrogenase (S-ADH) immobilized membrane onto fiber-optic NADH measurement system. This system utilizes ultraviolet light emitting diode as excitation source. Acetone vapor measured the fluorescence of consumption enzymatic S-ADH. A phosphate buffer that contained oxidized circulated into rinse products and excessive substrates from optode; thus, enables real-time monitoring photomultiplier tube detects change in emitted NADH. relationship between intensity concentration identified be 20 ppb 5300 ppb. encompasses range found healthy people those suffering disorders carbohydrate metabolism. Then, used exhaled before after meal. When sensing region exposed breath, decreased reached saturation immediately. it returned initial state upon cessation flow. anticipate its future use non-invasive analytical tool for assessment lipid metabolism exercise, fasting diabetes mellitus.