Accuracy of the Gaussian Point Spread Function model in 2D localization microscopy

Accuracy of the gaussian point spread function model in 2D localization microscopy.

The gaussian function is simple and easy to implement as Point Spread Function (PSF) model for fitting the position of fluorescent emitters in localization microscopy. Despite its attractiveness the appropriateness of the gaussian is questionable as it is not based on the laws of optics. Here we study the effect of emission dipole orientation in conjunction with optical aberrations on the local...

Multicolour localization microscopy by point-spread-function engineering.

Super-resolution microscopy has revolutionized cellular imaging in recent years1-4. Methods relying on sequential localization of single point emitters enable spatial tracking at ~10-40 nm resolution. Moreover, tracking and imaging in three dimensions is made possible by various techniques, including point-spread-function (PSF) engineering5-9 -namely, encoding the axial (z) position of a point ...

Algorithm for 3d Localization Microscopy Using Realistic Modeling of the Point Spread Function by Phase Retrieval

the norms of localization in translating persian multimodal texts: the case of videogame demos

abstract هنجارهای بومی سازی در ترجمه متون چندوجهی فارسی:مورد دموهای بازیهای کامپیوتری چکیده اهداف عمده مطالعه حاضر به سه دسته تقسیم میشوند: 1) بررسی مشکلات احتمالی ترجمه دموهای (فیلمهای) بازیهای کامپیوتری،2) تعیین هنجارهای بومی سازی در ترجمه دموهای (فیلمهای) بازیهای کامپیوتری و 3) تعیین ایدئولوژیهایی که این هنجارها در جامعه نشان میدهند. به این منظور، ابتدا، مجموعه ای ازدموهای (فیلمهای) ب...

Point spread function engineering in confocal scanning microscopy

Confocal scanning microscopes are imaging systems that are mainly featured by their unique depth-discrimination capacity when imaging three-dimensional objects. Along the past few years, our research group has done several attempts to improve their axial resolution by means of the so-called point-spread-function (PSF) engineering method. That is, by designing diffractive elements that properly ...