Accumulation ofO-Acetyl-l-homoserine, an Intermediate in Methionine Biosynthesis, by Methionine Auxotrophs ofArthrobacterandBacillusSpecies
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منابع مشابه
Homocysteine accumulation causes a defect in purine biosynthesis: further characterization of Schizosaccharomyces pombe methionine auxotrophs.
Methionine synthase (EC2.1.1.14) catalyses the final step in methionine synthesis, i.e. methylation of homocysteine. A search of the Schizosaccharomyces pombe genomic database revealed a gene designated SPAC9.09, encoding a protein with significant homology to methionine synthase. Disruption of SPAC9.09 caused methionine auxotrophy, and thus the gene was identified as a methionine synthase and ...
متن کاملBiosynthesis of methionine in liver.
Conversion of 1 carbon precursors to methionine methyl groups in a cell-free preparation was first demonstrated by Berg in this laboratory (2). Berg inactivated a pigeon liver extract by Dowex I-Cltreatment and dialysis and found that it could be activated by a boiled extract of pigeon liver. Methionine methyl formation in dialyzed liver estracts has been activated recently by mixtures of known...
متن کاملResponse of Aerobacter aerogenes methionine auxotrophs to adenine thiomethyl compounds.
It has been shown that many microorganisms produce thiomethyl adenosine from methionine (Smith and Schlenk, 1952). This prompted biochemical studies on the relationship between methionine and thiomethyl adenosine, which resulted in the discovery of methionyl adenosine as the key intermediate (Catoni, 1952; Schlenk, 1952). Replacement of methionine by thiomethyl adenosine has not been demonstrat...
متن کاملSymbiotic characteristics of cysteine and methionine auxotrophs of Sinorhizobium meliloti.
Twenty one cysteine and 13 methionine auxotrophs of Sinorhizobium meliloti Rmd201 were obtained by random mutagenesis with transposon Tn5. The cysteine auxotrophs were sulfite reductase mutants and each of these auxotrophs had a mutation in cysI/cysJ gene. The methionine auxotrophs were metA/metZ, metE and metF mutants. One hundred per cent co-transfer of Tn5-induced kanamycin resistance and au...
متن کاملBiosynthesis of ethylene. 4-methylmercapto-2-oxobutyric acid: an intermediate in the formation from methionine.
The enzyme responsible for the conversion of methionine into a precursor of ethylene in cauliflower florets is a transaminase. The formation of 4-methyl-mercapto-2-oxobutyric acid by this enzyme has been shown. The oxo acid stimulates the synthesis of ethylene when added to floret tissue, and tracer experiments have shown that (14)C is incorporated into ethylene from the labelled oxo acid. The ...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1969
ISSN: 0002-1369
DOI: 10.1080/00021369.1969.10859522