Abortive Infection of Sporulating Bacillus subtilis 168 by φ2 Bacteriophage

Abortive infection of sporulating Bacillus subtilis 168 by phi 2 bacteriophage.

Bacteriophage phi2 is unable to replicate in Bacillus subtilis 168. Although some phage deoxyribonucleic acid (DNA) synthesis can occur, the DNA made is not biologically active and sedimentation analysis reveals that it is smaller in size than that of mature DNA or DNA isolated from phi2-infected permissive hosts. Messenger ribonucleic acid hybridizable with phi2 DNA is also synthesized in phi2...

Bacteriophage Deoxyribonucleate Infection of Competent Bacillus Subtilis.

Reilly, Bernard E. (Western Reserve University, Cleveland, Ohio), and John Spizizen. Bacteriophage deoxyribonucleate infection of competent Bacillus subtilis. J. Bacteriol. 89:782-790. 1964.-Phenol extracts of the Bacillus subtilis bacteriophages phi1, phi25, and phi29 contained infectious deoxyribonucleic acid. The infectivity was destroyed by catalytic amounts of deoxyribonuclease but not by ...

Suppressor system in Bacillus subtilis 168.

Multiple auxotrophic strains of Bacillus subtilis 168 were tested for joint one-step reversion of two or more auxotrophic markers to the wild-type phenotype. Mu8u5u5, a strain requiring leucine, methionine, and threonine, yielded revertants that grew without added methionine or threonine and proved to have a suppressor gene. When transferred by transformation with deoxyribonucleic acid, this su...

Bacillus subtilis 168 4 5 6

Transduction in Bacillus subtilis by bacteriophage SPP1.

Lysates of the virulent bacteriophage SPP1 were shown to be capable of mediating generalized transduction. Suppressible mutants of this bacteriophage (sus) were capable of transduction at a lower multiplicity of infection than virulent SPP1. Linkage analysis demonstrated that bacteriophage SPP1 transduced segments of the genome equal in size to that transferred by SP10. This bacteriophage shoul...