ABO genotyping by polymerase chain reaction-restriction fragment length polymorphism
نویسندگان
چکیده
منابع مشابه
The prevalence of cryptosporidiosis in Turkish children, and genotyping of isolates by nested polymerase chain reaction-restriction fragment length polymorphism.
OBJECTIVE To verify the incidence of cryptosporidiosis among Turkish elementary school students. METHODS This study was conducted in the Department of Parasitology, Faculty of Medicine, Ege University, Turkey during a 3-month period in 2006. We assessed the fecal samples of 707 children using modified acid-fast and phenol-auramine staining followed by modified Ritchie concentration method. Al...
متن کاملWhole-blood polymerase chain reaction and restriction fragment length polymorphism: a simplified method by microwave irradiation.
OBJECTIVES The aim of the present study was to develop a simple, quick and cheap method to process whole-blood samples for the molecular techniques polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) without the use of expensive reagents or sophisticated machines. MATERIALS AND METHODS Venous whole-blood samples were collected from 40 individuals. The samples w...
متن کاملMolecular identification of Giardia duodenalis in Ecuador by polymerase chain reaction-restriction fragment length polymorphism
The aim of this study was to determine the genetic diversity of Giardia duodenalis present in a human population living in a northern Ecuadorian rain forest. All Giardia positive samples (based on an ELISA assay) were analysed using a semi-nested polymerase chain reaction-restriction fragment length polymorphism assay that targets the glutamate dehydrogenase (gdh) gene; those amplified were sub...
متن کاملIdentification of screwworm species by polymerase chain reaction-restriction fragment length polymorphism.
Restriction fragment length polymorphisms in polymerase chain reaction amplified fragments (PCR-RFLP) of mitochondrial DNA were used to differentiate species of New World screwworms (Diptera: Calliphoridae). Twenty-seven restriction enzymes were screened on five regions of mtDNA. Eleven restriction fragment length patterns differentiated New World screwworm, Cochliomyia hominivorax (Coquerel), ...
متن کاملIdentification of Dermatophytes by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Analysis of Metalloproteinase-1
BACKGROUND Transgenic research on metalloproteinase-1 is an emerging field in the area of plant molecular biology. The new method reported here can similarly be applied in fungal molecular biology to identify different dermatophytes. Our method is more accurate than traditional methods such as molecular analyses. OBJECTIVE To identify Trichophyton rubrum, T. mentagrophytes var. mentagrophytes...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Immunohematology
سال: 2020
ISSN: 0894-203X,1930-3955
DOI: 10.21307/immunohematology-2019-768