Abiotic stress triggers ROS-mediated programmed cell death in banana (Musa sp., AAA group, Cavendish sub-group) fruit

Degradative processes of fleshy fruit adapt depending on environmental conditions. Exposure banana to abiotic stress during ripening accelerates degradative that resemble Programmed Cell Death (PCD). This research evaluated the connection between Reactive Oxygen Species (ROS) production and PCD over-ripening after exposure at pre-climacteric phase (before exogenous ethylene treatment). Mature-green exposed 1 °C for 7 d exhibited severe chilling damage. During over-ripening, epidermis cells in chilled peel developed evident browning since 2 compared non-chilled peel, as well loss cell structure accelerated collapse. evaluation period H2O2 was higher (˃31% maximum) than (<26% whereas O2•− •OH equally increased both tissue until 10 d, (100% by end overripening. Nuclease activity 0 reaching values 255.9 units kg−1 fresh weight s−1 12 while from 212.9 s−1. Similarly, protease ranging 1800 152,814 protein However, levels DNA degradation or tailing were 6 once yellow, remained low tissue, never tissue. Our results demonstrated an alteration metabolism due dehydration, enzymatic oxidative Therefore, capacity maintain redox homeostasis, showed a necrotic form ROS-mediated PCD.