ABG of microtubule assembly

The Kinetics of Microtubule Assembly

A model describing the nucleation and assembly of purified tubulin has been developed. The novel feature of this model is a two stage nucleation process to allow the explicit inclusion of the two-dimensional nature of the early stages of microtubule assembly. In actin assembly the small starting nucleus has only one site for subunit addition as the two-stranded helix is formed. In contrast, mic...

Rapid Microtubule Self-Assembly Kinetics

Microtubule assembly is vital for many fundamental cellular processes. Current models for microtubule assembly kinetics assume that the subunit dissociation rate from a microtubule tip is independent of free subunit concentration. Total-Internal-Reflection-Fluorescence (TIRF) microscopy experiments and data from a laser tweezers assay that measures in vitro microtubule assembly with nanometer r...

Microtubule assembly in growing dendrites.

Dendritic microtubules (MTs) are nonuniform with respect to polarity orientation, with roughly equal proportions having a plus-end-distal or minus-end-distal orientation. In the present studies, we have microinjected biotin-labeled tubulin (Bt-tub) into cultured sympathetic neurons extending dendrites to explore the contribution of MT assembly to the elaboration and maintenance of the dendritic...

Effects of Inhibition of Microtubule Assembly

Dr. Mundy is the recipient of a Faculty Research Award (FRA-148) from the American Cancer Society. Dr. Mundy's present address is Divison ofEndocrinology and Metabolism, Department of Medicine, University ofTexas Health Science Center, San Antonio, Tex. Received for publication 23 April 1979 and in revised form 29 August 1980. INTRODUCTION Osteolytic bone metastases are a frequent complication ...

ABG made easy.