AB0345 Th9 CELLS AND THEIR ASSOCIATED CYTOKINES: THE PROBABLE PLAYERS IN SYSTEMIC LUPUS ERYTHEMATOSUS (SLE) AND TYPE 1 DIABETES MELLITUS (T1DM)

Background: SLE is an autoimmune disease (AID) of unknown origin. Several factors can contribute to immune dysfunction in SLE. Interleukin 9 (IL9) a newly emerging T cell-derived factor preferentially expressed by CD4+T cells: helper (Th9) IL9 targets different cell lineages, and the development allergic & AIDs Whether abnormal expression secretion are present patients (pts) still unidentified. It also unclear whether exerts main proinflammatory or anti-inflammatory activities T1DM characterized inflammation pancreatic islets Langerhans. Insulitis progresses over time β cells become destroyed then clinical DM established. regarded as cell-driven AID Objectives: Evaluation CD4+ IL9+ level pts compared both healthy subjects with another AID: T1DM.Also, evaluate correlation these expressions features, laboratory parameters activity Methods: The study included: Group I 25 fulfilling SLICC classification criteria divided into 2 subgroups (gps) according index (SLEDAI) IA: 20 mild moderate (<12) IB:5 severe (>12) recruited from rheumatology clinic internal medicine ward (Rheumatology unit), Main University Hospital, Alexandria. II 15 individuals first control gp. III American Diabetes Association second All were subjected history taking, examination,laboratory investigations: CBC,LFT,KFT,ESR,CRP,ANA,Anti-dsDNA,Th9 detection flowcytometry serum ELISA Results: There was no statistical difference between all gps regards age sex but significant increased ESR controls p< 0.001 p=0.001 Th9 highly pts, range 0.13-4.54% mean ±SD=1.50 ± 1.47% than gps. In ranged 0.0-1.29% 0.37 ±0.52%, while 0.03 2.13% 0.67 0.59%. A high found p=0.001, insignificant rise seen p=0.157. increase SLE: 3.74 ±1.15% 0.94±0.88% p=<0.001 pts: 42.83± 23.98 pg/ml 8.54±13.27, 29.17±16.09 pg/ml. normal p<0.001 p=0.294. ds p<0.001.A direct SLEDAI/105 damage p=0.040 not p=0.053 relation manifestations duration. correlations p=0.046 CRP p=0.025,a p=0.033, Th9&IL9 anti-dsDNA p=0.593& 0.4 Significant glycemic profile. levels significantly elevated p=0.033 % p=0.025, 0.046 Conclusion: pts; controls. percentages directly correlated activity. controls,but they less This suggests important role pathogenesis References: [1]Tahernia L et al. Cytokines their possible therapeutic opportunities. Rheum Res 2017 Disclosure Interests: None declared