AB0182 CONDITIONED MEDIA FROM PRIMARY OA CHONDROCYTES AFFECT THE BEHAVIOUR OF PHEOCHROMOCYTOMA PC 12 CELLS

Background Osteoarthritis (OA) is the most common joint disease characterized by progressive degradation of articular cartilage, synovial hyperplasia, bone remodeling and angiogenesis [1]. Symptomatic painful OA leads to psychological distress significant restrictions in daily living. Shortening work life accompanied an exponential rise healthcare costs causes become a major socio-economic burden [2]. Although, induced pain represents frequent cause chronic pain, its mechanisms are poorly understood treatments not satisfactory [3]. Objectives It great interest investigate whether OA-induced changes chondrocyte metabolism can affect neurons. Neurons with increased expression neurogenic markers could contribute enhanced perception. An investigation influence conditioned media from primary chondrocytes on PC12 cells may serve elucidate interactions. Methods cells, have been treated (pCH-OA) tested for RNA sequencing. Expression nestin, microtubule-associated protein 2 (Map2) tyrosine hydroxylase (TH) after treatment were analyzed qPCR. Screening relevant factors was performed using cytokine proteome profiler array. Electrophysiological measurements patch clamp method rat dorsal root ganglia neurons (DRGs) specific cytokines. Results sequencing showed profile when pCH-OA HC control compared standard differentiation growth media, respectively. Specific qPCR neuronal Map2, Th increase tested. In particular, Map2 TH almost all (n=14). array high levels IL6, IL8, MCP1 different patients. Treatment DRGs interleukin cocktail altered electrophysiological behavior patch-clamp technique provided first evidence that might secrete neuromodulatory substances. Conclusion Changes caused identification responsible metabolites should help shed more light process sensitization generation development detail at molecular level. addition, results also suggest there individual responses This information lays groundwork explaining why differences perception vary among patients phenotypes OA. References [1]Litwic, A., Edwards, MH., Dennison, EM., Cooper, C. Epidemiology osteoarthritis. Br Med Bull. 2013 , 105:185-99. [2]Safiri, S., et al. Global, regional national osteoarthritis 1990-2017: systematic analysis Global Burden Disease Study 2017. Ann Rheum Dis . 2020 79(6):819-828. [3]Eitner, Hofmann, GO., Schaible, HG. Mechanisms Osteoarthritic Pain. Studies Humans Experimental Models. Front Mol Neurosci 2017 3;10:349. Acknowledgements: NIL. Disclosure Interests None Declared.