A unified mechanism for plant polyketide biosynthesis derived from in silico modeling

Engineering Microbes for Plant Polyketide Biosynthesis

Polyketides are an important group of secondary metabolites, many of which have important industrial applications in the food and pharmaceutical industries. Polyketides are synthesized from one of three classes of enzymes differentiated by their biochemical features and product structure: type I, type II or type III polyketide synthases (PKSs). Plant type III PKS enzymes, which will be the main...

The biosynthesis of polyketide-derived mycotoxins.

The biosynthesis of a few representative polyketide-derived mycotoxins is reviewed: patulin, citrinin, diplosporin, ochratoxin A, maltoryzine, xanthomegnin, cytochalasans, ergochromes, zearalenone, citreoviridin, and the aflatoxins. Particular attention is given to the biosynthetic sequence: acetate----averufin----versiconal acetate----versicolorin A----sterigmatocystin----aflatoxin B1.

Dissecting complex polyketide biosynthesis

Numerous bioactive natural products are synthesised by modular polyketide synthases. These compounds can be made in high yield by native multienzyme assembly lines. However, formation of analogues by genetically engineered systems is often considerably less efficient. Biochemical studies on intact polyketide synthase proteins have amassed a body of knowledge that is substantial but still incomp...

Novel Intermolecular Iterative Mechanism for Biosynthesis of Mycoketide Catalyzed by a Bimodular Polyketide Synthase

In recent years, remarkable versatility of polyketide synthases (PKSs) has been recognized; both in terms of their structural and functional organization as well as their ability to produce compounds other than typical secondary metabolites. Multifunctional Type I PKSs catalyze the biosynthesis of polyketide products by either using the same active sites repetitively (iterative) or by using the...

Reactive Heterocycles for Examining Polyketide Biosynthesis