A type II restriction endonuclease of Streptococcus thermophilus ST117
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چکیده
منابع مشابه
Covalent joining of the subunits of a homodimeric type II restriction endonuclease: single-chain PvuII endonuclease.
The PvuII restriction endonuclease has been converted from its natural homodimeric form into a single polypeptide chain by tandemly linking the two subunits through a short peptide linker. The arrangement of the single-chain PvuII (sc PvuII) is (2-157)-GlySerGlyGly-(2-157), where (2-157) represents the amino acid residues of the enzyme subunit and GlySerGlyGly is the peptide linker. By introduc...
متن کاملThe Type II restriction endonuclease MvaI has dual specificity
The MvaI restriction endonuclease cuts 5'-CC↓AGG-3'/5'-CC↑TGG-3' sites as indicated by the arrows. N4-methylation of the inner cytosines (C(m4)CAGG/C(m4)CTGG) protects the site against MvaI cleavage. Here, we show that MvaI nicks the G-strand of the related sequence (CCGGG/CCCGG, BcnI site) if the inner cytosines are C5-methylated: C(m5)C↓GGG/CC(m5)CGG. At M.SssI-methylated SmaI sites, where tw...
متن کاملStreptococcus thermophilus.
Streptocus tmophilus, as a species designation (Orla-Jensen, 1919), has included those members of the nonhemolytic streptococci that possess the unusual combination of characteristics of extreme salt sensitivity, nonobligate thermophily, a rigorous fermentative pattern with respect to certain sugars (Sherman, 1937), and, based upon attempts to culture on synthetic media, a notoriety for nutriti...
متن کاملHjc resolvase is a distantly related member of the type II restriction endonuclease family.
Hjc resolvase is an archaeal enzyme involved in homologous DNA recombination at the Holliday junction intermediate. However, the structure and the catalytic mechanism of the enzyme have not yet been identified. We performed database searching using the amino acid sequence of the enzyme from Pyrococcus furiosus as a query. We detected 59 amino acid sequences showing weak but significant sequence...
متن کاملType II restriction endonuclease R.KpnI is a member of the HNH nuclease superfamily.
The restriction endonuclease (REase) R.KpnI is an orthodox Type IIP enzyme, which binds to DNA in the absence of metal ions and cleaves the DNA sequence 5'-GGTAC--C-3' in the presence of Mg2+ as shown generating 3' four base overhangs. Bioinformatics analysis reveals that R.KpnI contains a betabetaalpha-Me-finger fold, which is characteristic of many HNH-superfamily endonucleases, including hom...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1991
ISSN: 0378-1097
DOI: 10.1016/0378-1097(91)90212-s