A Tribe for RNA

A Modified Orcinol Reaction for RNA Determination

TRIBE: Hijacking an RNA-Editing Enzyme to Identify Cell-Specific Targets of RNA-Binding Proteins

RNA transcripts are bound and regulated by RNA-binding proteins (RBPs). Current methods for identifying in vivo targets of an RBP are imperfect and not amenable to examining small numbers of cells. To address these issues, we developed TRIBE (targets of RNA-binding proteins identified by editing), a technique that couples an RBP to the catalytic domain of the Drosophila RNA-editing enzyme ADAR ...

A Mutable Tribe

VSMC signaling, tunica media formation, and VSMC dif-fungi lay in using them, with the visible morphology of their mycelium, asexual dispersal systems, and fruiting ferentiation. The remaining five chapters of the book bodies, for studies of development. J.T. Bonner and his provide details on the formation of blood vessels in students used the slime mold Dictyostelium dicoideum selected vascula...

a modified orcinol reaction for rna determination

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HyperTRIBE identifies many more in vivo targets of a RNA-binding protein than TRIBE

Most current methods to identify cell-specific RNA binding protein (RBP) targets require analyzing an extract, a strategy that is problematic with small amounts of material. To address this issue, we developed TRIBE, a genetic method that expresses an RBP of interest fused to the catalytic domain of the RNA editing enzyme ADAR. TRIBE therefore performs Adenosine-to-Inosine editing on candidate ...