A Strategy for Synthesis of Pathogenic Human Immunoglobulin Free Light Chains in E. coli
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منابع مشابه
A Strategy for Synthesis of Pathogenic Human Immunoglobulin Free Light Chains in E. coli
Monoclonal immunoglobulin light chains are normally synthesized in excess compared to the heavy chain partners and can be detected in serum and urine ("free" LC). Occasionally free LC are per se cause of organ toxicity, as in free LC-related disorders. In AL amyloidosis, the most common of these conditions, free LC with peculiar biophysical properties related to their primary structure damage t...
متن کاملMeasurement of immunoglobulin free light chains in serum.
but the active/latent isoform proportions were similar (Fig. 1A). Platelet aggregation during clotting (6) may have caused these differences. MMP expression was lower in K 2 E plasma than in LH plasma [14 (3) vs 25 (4) g/L; P Ͻ0.05; Fig. 1A, lane 4 vs lane 5]. The concentrations of MMP-9 forms decreased significantly with increasing amounts of K 2 E during PB collection, whereas MMP-2 was incr...
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Several newly arising human antibodies are polyreactive, but in normal individuals the majority of these potentially autodestructive antibodies are removed from the repertoire by receptor editing or B cell deletion in the bone marrow. To determine what proportion of naturally arising autoantibodies can be silenced by immunoglobulin (Ig) light chain receptor editing, we replaced the light chains...
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BACKGROUND Intrathecal immunoglobulin synthesis is observed in several inflammatory disorders of the central nervous system, but its detection by current laboratory tests is either tedious or relatively insensitive. We assessed the diagnostic accuracy of an assay for kappa free light chains (kappaFLC) in cerebrospinal fluid (CSF) and serum, and compared it with traditional tests for intrathecal...
متن کاملFree immunoglobulin light chains in connective tissue diseases.
The basic immunoglobulin subunit consists of two heavy and two light chains held together by covalent disulphide bonds. In most cases of myeloma and Waldenstrom's disease there is an excess synthesis of light over heavy chains, resulting in Bence Jones proteinaemia and proteinuria. Furthermore, in some cases ("light chain disease"), there is absence of detectable heavy chain synthesis, and the ...
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ژورنال
عنوان ژورنال: PLoS ONE
سال: 2013
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0076022