A Step Towards Optimization of the qNMR Workflow: Proficiency Testing Exercise at an GxP-Accredited Laboratory

Abstract Quantitative nuclear magnetic resonance (qNMR) is considered as a powerful tool for multicomponent mixture analysis well the purity determination of single compounds. Special attention currently paid to training operators and study directors involved in qNMR testing. To assure that only qualified personnel are used sample preparation at our GxP-accredited laboratory, weighing test was proposed. Sixteen participants performed six-fold binary dibutylated hydroxytoluene (BHT) 1,2,4,5-tetrachloro-3-nitrobenzene (TCNB). evaluate quality data analysis, all spectra were evaluated manually by expert using in-house developed automated routine. The results revealed mean values comparable both evaluation approaches free systematic error. However, resulted an approximately 20% increase precision. same findings 32 compounds pharmaceutical industry. Weighing mixtures methodology can be recommended efficient tools evaluating staff proficiency. method significantly increases throughput precision routine measurements extends application scope qNMR.