A stalled-ribosome rescue factor Pth3 is required for mitochondrial translation against antibiotics in Saccharomyces cerevisiae
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چکیده
منابع مشابه
Cisplatin cytotoxicity is dependent on mitochondrial respiration in Saccharomyces cerevisiae
Objective(s): To understand the role of mitochondrial respiration in cisplatin sensitivity, we have employed wild-type and mitochondrial DNA depleted Rho0 yeast cells. Materials and Methods: Wild type and Rho0 yeast cultured in fermentable and non-fermentable sugar containing media, were studied for their sensitivity against cisplatin by monitoring growth curves, oxygen consumption, pH changes ...
متن کاملIdentification of residues required for stalled-ribosome rescue in the codon-independent release factor YaeJ
The YaeJ protein is a codon-independent release factor with peptidyl-tRNA hydrolysis (PTH) activity, and functions as a stalled-ribosome rescue factor in Escherichia coli. To identify residues required for YaeJ function, we performed mutational analysis for in vitro PTH activity towards rescue of ribosomes stalled on a non-stop mRNA, and for ribosome-binding efficiency. We focused on residues c...
متن کاملCbp1 is required for translation of the mitochondrial cytochrome b mRNA of Saccharomyces cerevisiae.
Expression of the yeast mitochondrial cytochrome b gene (COB) is controlled by at least 15 nuclear-encoded proteins. One of these proteins, Cbp1, is required for COB mRNA stability. Delta cbp1 null strains fail to accumulate mature COB mRNA and cannot respire. Since Delta cbp1 null strains lack mature COB transcripts, the hypothesis that Cbp1 also plays a role in translation of these mRNAs coul...
متن کاملcisplatin cytotoxicity is dependent on mitochondrial respiration in saccharomyces cerevisiae
objective(s): to understand the role of mitochondrial respiration in cisplatin sensitivity, we have employed wild-type and mitochondrial dna depleted rho0 yeast cells. materials and methods: wild type and rho0 yeast cultured in fermentable and non-fermentable sugar containing media, were studied for their sensitivity against cisplatin by monitoring growth curves, oxygen consumption, ph changes ...
متن کاملDbp10p, a putative RNA helicase from Saccharomyces cerevisiae, is required for ribosome biogenesis.
Ribosome biogenesis requires, in addition to rRNA molecules and ribosomal proteins, a multitude of trans-acting factors. Recently it has become clear that in the yeast Saccharomyces cerevisiae many RNA helicases of the DEAD-box and related families are involved in ribosome biogenesis. Here we show that the previously uncharacterised open reading frame YDL031w (renamed DBP10 for DEAD-box protein...
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ژورنال
عنوان ژورنال: Communications Biology
سال: 2021
ISSN: 2399-3642
DOI: 10.1038/s42003-021-01835-6