A Specific Thin Layer Chromatography Method for the Identification and Separation of Medium Chain Acylglycerols

A preparative high performance liquid chromatography method for separation of lecithin: comparison to thin-layer chromatography.

We have developed a high performance liquid chromatography (HPLC) method to separate lecithin from other phospholipid classes and to obtain lecithin from biologic materials. The separation was performed on a preparative 10-micron Spherisorb column with an optimized solvent system consisting of the following components: acetonitrile, isopropanol, methanol, water, and trifluoroacetic acid. The ad...

A Simple Method for the Separation of Minute Amounts of Tissue Lipids by Thin-layer Chromatography and Gas-liquid Chromatography.

Identification of Barbiturates by Thin-layer Chromatography.

in cases of overdose and identification of the actual barbiturate concerned may be impossible because of the closely similar Rf values of this group. Jackson (1960) described treatment with hot sulphuric acid before paper chromatography to distinguish between barbiturates of similar chromatographic mobility. The inert nature of the silica gel supporting medium used in thin-layer separations ena...

Separation of Cholesterol and Demosterol by Thin-layer Chromatography.

Separation of Long-chain Bases by Thin-layer Chromatography; Instability of Sphingosine.

Purified sphingosine was resolved into dihydrosphingosine, sphingosine, and a fast-moving component by thin-layer chromatography on Silica Gel G. The fast-moving component was generated by the adsorbent, and consisted of a mixture of the cationic forms of dihydrosphingosine and sphingosine. Other sphingosine derivatives gave rise to similar artifacts. Sphingosine formed two degradation products...