A Species-Specific Polymerase Chain Reaction Assay for Rapid Detection of Phytophthora nicotianae in Irrigation Water
نویسندگان
چکیده
منابع مشابه
Polymerase chain reaction assay targeting nox gene for rapid identification of Brachyspira canis in dogs
Genus Brachyspira,as Gram negative anaerobic bacteria, colonize in dogs intestine. The aim of the current study was to determine the prevalence of Brachyspira spp. for the first time in Iran and rapid identification of Brachyspira spp. in dogs by a new designment of a species-specific primer set for B. canis. One hundred fifty-one fecal samples were obtained ...
متن کاملrapid and specific polymerase chain reaction-enzyme linked immunosorbent assay for detection of escherichia coli lt toxin from clinical isolates
methods this experimental study was conducted on iranian children communities from may to november 2014. forty stool samples were obtained from laboratories and investigated for heat-labile toxin (lt). specific primers were designed and the dig -labeled pcr products were bounded to streptavidin-coated wells of a microtiter plate and detected by anti-dig-peroxidase conjugate. an internal biotin-...
متن کاملrapid detection of isoniazid resistance in mycobacterium tuberculosis by a single multiplex allele-specific polymerase chain reaction assay
objective: global surveillance has shown that drug resistant (dr) tuberculosis (tb) is widespread. prompt detection of mycobacterium tuberculosis drug resistance is essential for effective control of tb. the most frequent mutations associated with isoniazid (inh) resistance in mycobacterium are substitutions at codons 315 in the katg gene and the maba-inha promoter region (−15). this survey eva...
متن کاملPolymerase chain reaction method for the rapid detection of virulent Shigella spp.
Bacillary dysentery, or shigellosis, is a disease of humans in which the colonic epithelium is invaded by bacteria and subjected to inflammatory destruction. The aim of this study was to develop a polymerase chain reaction(PCR) test for detection of virulent Shigella spp.. For this purpose, the primers were designed to amplify a 526-bp internal region of the Shigella spp. icsA gene, which encod...
متن کاملA genus- and species-specific nested polymerase chain reaction malaria detection assay for epidemiologic studies.
A nested polymerase chain reaction (PCR) assay that uses Plasmodium genus-specific primers for the initial PCR (nest 1) amplification and either genus- or species-specific primers for the nest 2 amplifications was tested on laboratory and field samples. With in vitro cultured Plasmodium falciparum-infected blood samples, it was capable of detecting six parasites/microl of blood using DNA prepar...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Phytopathology®
سال: 2003
ISSN: 0031-949X,1943-7684
DOI: 10.1094/phyto.2003.93.7.822