A Simultaneous Determination of Benzophenone and Camphor UV-Filters, Together with Metabolites of Polycyclic Aromatic Hydrocarbons, in Human Urine

Abstract By monitoring biomarkers in urine, personal exposure to selected chemical substances can be assessed. In this paper, we describe the of 7 benzophenone and 2 camphor UV-filters 11 metabolites polycyclic aromatic hydrocarbons (OH-PAHs) 454 urine samples collected from mothers new-borns an industrial (Karvina) reference area (Ceske Budejovice) over winter summer seasons. The analytical method consisted enzymatic hydrolysis, liquid–liquid extraction (ethyl acetate), purification by dispersive solid-phase (Z-Sep sorbent) ultra-high performance liquid chromatography coupled with tandem mass spectrometry. Benzophenone-1 (BP-1), naphthalen-2-ol (2-OH-NAP), fluoren-2-ol (2-OH-FLUO) phenanthren-2-ol (2-OH-PHEN) were determined all analysed. Benzophenone-3 (BP-3) 2-OH-NAP found at highest concentrations (medians 5.95- 5.77-µg/g creatinine). Analytes 3-(4-methylbenzylidene)camphor (3-MBC), 3-benzylidenecamphor (3-BC), chrysen-6-ol (6-OH-CHRY) benzo(a)pyrene-3-ol (3-OH-BaP) not detected any sample. Median OH-PAHs compared across regions Czech Republic between their different countries. median measured our study similar Brazilian children these compounds mothers’ comparable Danish mothers. case OH-PAHs, new-born previous study, those reported Spanish women.