A simple culture method forBrassica hypocotyl protoplasts
نویسندگان
چکیده
منابع مشابه
A simple method for large-scale electrofusion and culture of plant protoplasts.
A simple method is described for the electrofusion of plant protoplasts. Protoplasts were aggregated in a radio-frequency field (10 V RMS, 0.5 MHz) for 15-30 s with an inter-electrode distance of 5 mm. They were then fused with a 300-V DC pulse. The protoplasts were able to divide after this treatment. A transferrable electrode permitted electrofusion of 1-ml volumes of culture in standard tiss...
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ژورنال
عنوان ژورنال: Plant Cell Reports
سال: 1985
ISSN: 0721-7714,1432-203X
DOI: 10.1007/bf00571310