A Region of the Ryanodine Receptor Critical for Excitation-Contraction Coupling in Skeletal Muscle

Role of the ryanodine receptor of skeletal muscle in excitation-contraction coupling.

In skeletal muscle, contraction is initiated by a depolarization of the transverse tubular membrane (t-tubule), which in turn signals the release of Ca from the sarcoplasmic reticulum (SR). A key protein involved in this process is the ryanodine receptor, an SR membrane protein of MW 450,000 that binds the alkaloid ryanodine with nanomolar affinity and is present exclusively at the junction bet...

Excitation-contraction coupling in skeletal muscle.

Triadin Binding to the C-Terminal Luminal Loop of the Ryanodine Receptor is Important for Skeletal Muscle Excitation–Contraction Coupling

Ca(2+) release from intracellular stores is controlled by complex interactions between multiple proteins. Triadin is a transmembrane glycoprotein of the junctional sarcoplasmic reticulum of striated muscle that interacts with both calsequestrin and the type 1 ryanodine receptor (RyR1) to communicate changes in luminal Ca(2+) to the release machinery. However, the potential impact of the triadin...

Excitation-contraction coupling in skeletal muscle of a mouse lacking the dihydropyridine receptor subunit gamma1.

1. In skeletal muscle, dihydropyridine (DHP) receptors control both Ca(2+) entry (L-type current) and internal Ca(2+) release in a voltage-dependent manner. Here we investigated the question of whether elimination of the skeletal muscle-specific DHP receptor subunit gamma1 affects excitation-contraction (E-C) coupling. We studied intracellular Ca(2+) release and force production in muscle prepa...

Local Control Model of Excitation–Contraction Coupling in Skeletal Muscle

This is a quantitative model of control of Ca release from the sarcoplasmic reticulum in skeletal muscle, based on dual control of release channels (ryanodine receptors), primarily by voltage, secondarily by Ca (Ríos, E., and G. Pizarro. 1988. 3:223-227). Channels are positioned in a double row array of between 10 and 60 channels, where exactly half face voltage sensors (dihydropyridine recepto...