A Recombinant Chimera Vaccine Composed of LTB and Mycoplasma hyopneumoniae Antigens P97R1, mhp390 and P46 Elicits Cellular Immunologic Response in Mice

Mycoplasma hyopneumoniae is the etiological agent of porcine enzootic pneumonia (EP), leading to a mild and chronic in swine. Relative control has been attained through active vaccination programs, but remains significant economic challenge swine industry. Cellular immunity plays key role prevention pneumonia. Therefore, development more efficient vaccine that confers strong against M. necessary. In this study, multi-antigen chimera (L9m6) was constructed by combining heat-labile enterotoxin B subunit (LTB) with three antigens (P97R1, mhp390, P46), its immunogenic antigenic properties were assessed murine model. addition, we compared effect individual administration multiple-fusion these antigens. The chimeric multi-fusion induced cellular immune responses high production IgG IgM antibodies hyopneumoniae. Collectively, our data suggested rL9m6 exhibits potential as viable candidate for