A rapid extraction method for glycogen from formalin-fixed liver

نویسندگان
چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Dna Extraction from Formalin Fixed Franciscana Tissues

– The present paper reports the extraction of DNA from formalin-fixed Pontoporia blainvillei tissues. Following the Vachot and Monerot (1996) protocol, fragmented DNA (300-700bp) was extracted from more than 95% of liver and muscle samples. DNA yield in liver samples was significantly higher than in muscle samples (4.574 ± 1.169μg DNA/mg versus 0.808 ± 0.297μg DNA/mg). Similar results were obta...

متن کامل

An Optimized Method of Metabolite Extraction from Formalin-Fixed Paraffin-Embedded Tissue for GC/MS Analysis

Formalin-fixed paraffin-embedded (FFPE) tissue specimens constitute a highly valuable source of clinical material for retrospective molecular studies. However, metabolomic assessment of such archival material remains still in its infancy. Hence, there is an urgent need for efficient methods enabling extraction and profiling of metabolites present in FFPE tissue specimens. Here we demonstrate th...

متن کامل

Nucleic Acids Extraction from Formalin-Fixed and Paraffin-Embedded Tissues

Formalin-fixed paraffin-embedded (FFPE) tissues are an important sample source for ret‐ rospective studies. Despite its ability to preserve proteins and cell morphology, formalin hinders molecular biology tests since it fragments and chemically modifies nucleic acids, especially RNA. Although several studies describe techniques that allow extracting nu‐ cleic acids from FFPE tissues, so far the...

متن کامل

DNA extraction from formalin-fixed, paraffin-embedded tissue.

Several DNA extraction methods have been used for formalin-fixed, paraffin-embedded tissues, with variable results being reported regarding the suitability of DNA obtained from such sources to serve as template in polymerase chain reaction (PCR)-based genetic analyses. We present a method routinely used for archival material in our laboratory that reliably yields DNA of sufficient quality for P...

متن کامل

A rapid RT-PCR method for detection of intact RNA in formalin-fixed paraffin-embedded tissues.

The molecular analysis of formalin-fixed and paraffin-embedded tissues has application in both investigative studies and clinical practice (1). It is now possible to analyze gene expression in formalin-fixed and paraffin-embedded tissues by in situ reverse transcriptase-polymerase chain reaction (in situ RT-PCR) (2). Degradation of RNA, however, can occur at various stages of tissue preparation...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Carbohydrate Polymers

سال: 2015

ISSN: 0144-8617

DOI: 10.1016/j.carbpol.2014.11.005