A rapid DNA isolation procedure from Petri dish grown clinical bacterial isolates
نویسندگان
چکیده
منابع مشابه
A rapid DNA isolation procedure from petri dish grown clinical bacterial isolates.
Gardnerella vaginalis, a pleomorphic Gram variable bacteria (1), with a Gram positive cell wall (2). that stains predominantly as Gram negative is associated in bacterial vaginosis (1). We have described a lysozyme-SDS-proteinase K method for the isolation of DNA from 250 ml Casman broth cultures requiring about 60 to 72 hours (3). However, this was not convenient for comparative restriction en...
متن کاملESBL - from petri dish to the patient.
Treatment of extended spectrum beta-lactamase (ESBL) producing strains of Enterobacteriaceae has emerged as a major challenge in hospitalised as well as community based patients. Infections due to ESBL producers range from uncomplicated urinary tract infection to life threatening sepsis. Although several reviews have been published in the literature about the detection and identification of the...
متن کاملA rapid procedure for the isolation of plasmid DNA from environmental bacteria.
The INSTA-MINI-PREP method, a rapid protocol for plasmid DNA extraction, was originally developed to prepare plasmid DNA from 1 to 3 ml miniprep Escherichia coli cultures. Direct extraction of plasmid DNA is achieved by a two-phase solution which is separated by centrifugation in the presence of the INSTA-PREP gel barrier material. This method has been successfully tested on various environment...
متن کاملIsolation, Identification and Partial Optimization of Novel Xylanolytic Bacterial Isolates from Bhilai-Durg Region, Chhattisgarh, India
Background: Plant biomass and agricultural waste products disposal is a serious problem in agriculture based countries. These wastes, usually rich in xylan can be satisfactorily converted to industrially important and useful products by efficient biotechnological application of potent xylanase producing bacteria which generally have high temperature and pH optima....
متن کاملUnderstanding mobility in a social petri dish
Despite the recent availability of large data sets on human movements, a full understanding of the rules governing motion within social systems is still missing, due to incomplete information on the socio-economic factors and to often limited spatio-temporal resolutions. Here we study an entire society of individuals, the players of an online-game, with complete information on their movements i...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1990
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/18.21.6462