A Rapid, Automated Enzymatic Fluorometric Assay for Determination of d -Arabinitol in Serum
نویسندگان
چکیده
منابع مشابه
A rapid, automated enzymatic fluorometric assay for determination of D-arabinitol in serum.
A rapid enzymatic fluorometric assay for measuring D-arabinitol in serum was developed using recombinant D-arabinitol dehydrogenase from Candida albicans (rArDH). rArDH was produced in Escherichia coli and purified by dye-ligand affinity chromatography. rArDH was highly specific for D-arabinitol, cross-reacting only with xylitol (4.9%) among all polyols tested. A Cobas Fara II centrifugal autoa...
متن کاملFluorometric enzymatic determination of total cholesterol in serum.
We describe a fluorometric enzymatic method for determining total serum cholesterol, based on hydrolysis of cholesterol esters to free cholesterol by cholesterol ester hydrolase (EC 3.1.1.13). The free cholesterol formed, as well as that initially present, is then oxidized by cholesterol oxidase (EC 1.1.3.6) to cholest-4-en-3-one with simultaneous production of hydrogen peroxide. The latter cat...
متن کاملA new, rapid fluorometric determination of retinol in serum.
A new procedure for the determination of retinol (vitamin A alcohol) in blood has been devised. It consists of direct measurement of the fluorescence of retinol (excitation 335 nm.; emission 458 nm.) in diluted serum, and is considerably more rapid than any previous method. Such measurements are feasible because the intensity of fluorescence of retinol bound to its transport protein (retinol-bi...
متن کاملAutomated fluorometric method for the determination of serum calcium.
A. FULLY automated nlethod for the (leterinination of serum calcium which is accurate, reproducible, and relatively simple seemed desirable. The additioll of the fluorometer (1) to the modular system of the AutoAnalyzer* suggested the search for a fluorometric calcium procedure. Accordingly, several substances known to complex with calcium were obtained and their fluorescent emission spectra in...
متن کاملAutomated enzymatic determination of sodium in serum.
An automated method based on the principles of flow-injection analysis is proposed for the enzymatic determination of sodium ion in serum. The method relies on the activation of beta-galactosidase by the analyte. The features of the proposed method include linear range between 1 and 1700 mumol/L, a sampling rate of 50 samples/h, a sample volume of 50 microL, and the absence of interferences fro...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2000
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.38.4.1439-1443.2000