A Radio-Immunoassay of 19-Nortestosterone Using Celite Column Chromatography

Urinary metabolites of administered 19-nortestosterone.

The reported high anabolic (myotrophic) and low androgenic activity of 19-nortestosterone and its esters in rodents (l-5) prompted an oxperiment to determine whether this substance was useful in human breast cancer as a palliative agent free from virilizing effects. As a result of this experiment it became possible to study its metabolism’ in a human subject. In view of the absence of the angul...

Production and characterisation of monoclonal antibodies against 19-Nortestosterone.

OBJECTIVE To produce anti-19-Nortestosterone (NT) monoclonal antibodies and identify their immunological characteristics. METHODS Hybridomas were prepared by fusing NS0 mouse myeloma cells with splenocytes isolated from immunized BALB/c mice. Noncompetitive and competitive indirect ELISA were employed to screen positive cell clones. A caprylic acid ammonium sulphate (CAAP) method was used to ...

Plasma concentrations of testosterone and 19-nortestosterone (nandrolone) in the nonracing intact male horse by liquid chromatography-mass spectrometry.

The Commonwealth of Pennsylvania regulates the use of anabolic and androgenic steroids by monitoring plasma samples obtained from equine athletes post competition. Plasma samples were chosen over urine because the pharmacological action of any drug is generally based on plasma concentration of the parent or active metabolite of the compound and not its concentration in urine. Furthermore, the c...

Column Liquid Chromatography

Separation of Somatropin and Its Degradation Products by High-Performance Liquid Chromatography Using a Reversed-Phase Polymeric Column

The accurate prediction of protein stability is one of the most challenging goals in protein formulation and delivery. In this study, a gradient RP-HPLC method is described for the separation of human growth hormone (hGH) variants as deamidated and oxidized forms. The methodology employed a polymeric poly (styrene-co-divinylbenzene) column and a 1mL/min flow rate of a linear gradient of 0.1% v/...