A quantitative assay for mouse granulocyte (CFU-g) and macrophage (CFU-m) precursors using plasma clots.
نویسندگان
چکیده
منابع مشابه
Treatment with Interferon- a Preferentially Reduces the Capacity for Amplification of Granulocyte-Macrophage Progenitors (CFU-GM) from Patients with Chronic Myeloid Leukemia but Spares Normal CFU-GM
The biological target for interferon (IFN)a in chronic myeloid leukemia (CML) is unknown, but one possibility is that amplification of granulocyte-macrophage colony-forming cells (CFU-GM) is reduced. Replating CFU-GM colonies and observing secondary colony formation provides a measure of CFU-GM amplification. Amplification of CML, but not normal, CFU-GM in vitro was significantly inhibited by I...
متن کاملImproved plasma culture system for production of erythrocytic colonies in vitro: quantitative assay method for CFU-E.
An improved plasma culture system is described for the production of erythrocytic colonies by mammalian adult hemopoietic cells in vitro under the influence of erythropoietin. The concentration of fetal calf serum in the medium used for dilution of the cells was critical for erythrocytic colony formation when low numbers of cells were plated. Optimal concentrations were found for plasma, fetal ...
متن کاملCFU-M-derived human megakaryocytes synthesize glycoproteins IIb and IIIa.
Human megakaryocytes have been shown by immunofluorescent techniques to express platelet glycoprotein IIb/IIIa antigen. We report evidence that megakaryocytes derived from human committed megakaryocytic progenitor cells in vitro (CFU-M) synthesize glycoproteins IIb and IIIa. Nonadherent light-density human bone marrow cells were cultured in human plasma and methylcellulose using conditions that...
متن کاملCFU - M - Derived Human Megakaryocytes Synthesize Glycoproteins lib and lila
Human megakaryocytes have been shown by immunofluorescent techniques to express platelet glycoprotein lIb/Illa antigen. We report evidence that megakaryocytes derived from human committed megakaryocytic progenitor cells in vitro (CFU-M) synthesize glycoproteins lIb and lIla. Nonadherent light-density human bone marrow cells were cultured in human plasma and methylcellulose using conditions that...
متن کاملTo the editor : CFU
We read with interest the article “Redefining endothelial progenitor cells via clonal analysis and hematopoietic stem/progenitor cell principles” by Yoder et al.1 The authors compared 2 types of colony assays that have been shown to identify endothelial progenitor cells (EPCs)2,3 and studied the function of the cells derived from these assay systems. They found that a commercially available kit...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1985
ISSN: 0022-1554,1551-5044
DOI: 10.1177/33.7.4008915