A quantitative assay for assessing allelic proportions by iterative gap ligation

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A quantitative assay for assessing allelic proportions by iterative gap ligation.

A variety of techniques are currently available for detecting point mutations in DNA. These techniques are frequently not sensitive enough to be applied as quantitative assays in evaluation of relative occurrence of alleles in cases of polymorphism or when variations in allelic gene expression are being evaluated at the level of RNA. We report here the establishment of an iterative gap ligation...

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ژورنال

عنوان ژورنال: Nucleic Acids Research

سال: 1998

ISSN: 1362-4962

DOI: 10.1093/nar/26.4.961