A protein multiplex microarray substrate with high sensitivity and specificity
نویسندگان
چکیده
منابع مشابه
Multiplex approaches in protein microarray technology.
The success of genome sequencing projects has provided the basis for systematic analysis of protein function and has led to a shift from the description of single molecules to the characterization of complex samples. Such a task would not be possible without the provision of appropriate high-throughput technologies, such as protein microarray technology. In addition, the increasing number of sa...
متن کاملComparison sensitivity and specificity temporal artery temperature measurement with axillary and nasopharyngeal methods in adult
Aims: temporal artery thermometers (TAT) of new tools for measuring body temperature, few studies have been conducted to evaluate the accuracy and reliability of thermometers and is still uncertain accuracy and reliability this thermometers.This study to evaluate the accuracy of the two methods of measuring body temperature by the thermometer axillary and temporal artery compared with nas...
متن کاملDNA analysis with multiplex microarray-enhanced PCR
We have developed a highly sensitive method for DNA analysis on 3D gel element microarrays, a technique we call multiplex microarray-enhanced PCR (MME-PCR). Two amplification strategies are carried out simultaneously in the reaction chamber: on or within gel elements, and in bulk solution over the gel element array. MME-PCR is initiated by multiple complex primers containing gene-specific, forw...
متن کاملSubstrate specificity of protein kinase C inhibitors.
In the August issue of TiPS, Epand and Lester analysed the effect of the physicochemical properties of the membrane on protein kinase C activity. In addition to those substrates discussed that require the presence of Ca2+ and phospholipids, other substrates can be phosphorylated in the absence of these activators. It is possible that this differential substrate requirement could be exploited ph...
متن کاملDesigning of a High-Sensitivity and Low-Background cDNA Microarray
The microarray is an efficient device to analyze the expression level of many genes at once. cDNA microarrays are a promising technique for elucidating and interpreting the mechanistic roles of genes in the pathogenesis of patient’s tissues. However there has not been available cDNA microarrays that be able to analyze with small amount of RNA for each target-tissue. Moreover, the possibility of...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Immunological Methods
سال: 2010
ISSN: 0022-1759
DOI: 10.1016/j.jim.2010.10.005