A preferable approach to clone hLIF cDNA from the genomic DNA

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A preferable approach to clone hLIF cDNA from the genomic DNA

Complementary DNA (cDNA) is valuable for investigating protein structure and function in the research of life science, but it is difficult to obtain by traditional reverse transcription. In this study, we employed a novel strategy to clone the human leukemia inhibitory factor (hLIF) gene cDNA from genomic DNA directly isolated from the mucous membrane of mouth. The hLIF sequence can be acquired...

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ژورنال

عنوان ژورنال: Electronic Journal of Biotechnology

سال: 2011

ISSN: 0717-3458,0717-3458

DOI: 10.2225/vol14-issue3-fulltext-10