A Potential Lead from Acacia nilotica (L.) Delile Against Hepatitis C virus - An In silico Approach

Hepatitis C virus infection is the leading cause of chronic liver disease and hepatocellular carcinoma. There no effective vaccine for hepatitis prevention despite fact that severalvaccinesare under development. Currently, Unites States Food drug administration approved combination drugs all genotypes would help to cure more quickly efficiently than ever before. However, high costs, development various side effects emergence resistant strains demand need new anti-viral treat different stages life cycle. Focussing candidate from herbal ingredients novel approach pharmaceutical science over past few decades. In this perspective, present study aimed investigate phytochemicals in Acacia nilotica (L.) Delile against non-structural protein3-4A serine protease. The N-terminal Protease domain protein3 along with protein4A protein responsible cleavage four polypeptide junctions’ viz., protein3-4A, protein4A-non-structural protein4B, protein4B-non-structural protein5A protein5A-5B are essential viral genome replication. Hence targeting blocks replication process. Here, silico molecular docking was executed estimate efficacy ofphytochemicals two inhibitors-Grazoprevir simeprevir as reference compoundsagainst selected target. Docking results revealed about six (+)-Catechin 5-Gallate, Acacetin, (+)-Mollisacacidin, Catechin, Acalinol A Chlorogenic acid better compounds hence hits. Further, hit molecules were filtered through analysing druglikeness properties, pharmacokinetics, medicinal chemistry friendliness including pan assay interference Brenk structural alerts, leadlikeness finally prediction potential toxicity toxic substructure ascertain a lead molecule. obtained current propose Acacetin molecule further vitro vivo study.