A Phosphorylation Tag for Uranyl Mediated Protein Purification and Photo Assisted Tag Removal
نویسندگان
چکیده
منابع مشابه
A Phosphorylation Tag for Uranyl Mediated Protein Purification and Photo Assisted Tag Removal
Most protein purification procedures include an affinity tag fused to either the N or C-terminal end of the protein of interest as well as a procedure for tag removal. Tag removal is not straightforward and especially tag removal from the C-terminal end is a challenge due to the characteristics of enzymes available for this purpose. In the present study, we demonstrate the utility of the divale...
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ژورنال
عنوان ژورنال: PLoS ONE
سال: 2014
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0091138