A patch-clamp study of mammalian platelets and their voltage-gated potassium current.
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چکیده
منابع مشابه
Voltage Clamp Fluorimetry Reveals a Novel Outer Pore Instability in a Mammalian Voltage-gated Potassium Channel
Voltage-gated potassium (Kv) channel gating involves complex structural rearrangements that regulate the ability of channels to conduct K(+) ions. Fluorescence-based approaches provide a powerful technique to directly report structural dynamics underlying these gating processes in Shaker Kv channels. Here, we apply voltage clamp fluorimetry, for the first time, to study voltage sensor motions i...
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embryonic stem cells are capable of differentiating to variety of cell tissues including cardiomyocytes. this developmental change is accompanied with a great deal of ion channel expression and functions. mouse stem cell derived cardiomyocytes were prepared and separated to yield isolated single cell suspension for cell current recording. in the present study some properties of the k + -current...
متن کاملVoltage clamp fluorimetry studies of mammalian voltage-gated K(+) channel gating.
VCF (voltage clamp fluorimetry) provides a powerful technique to observe real-time conformational changes that are associated with ion channel gating. The present review highlights the insights such experiments have provided in understanding Kv (voltage-gated potassium) channel gating, with particular emphasis on the study of mammalian Kv1 channels. Further applications of VCF that would contri...
متن کاملVoltage-Gated Potassium Channels
Physiol. Soc.. ESSN: 1548-9221. Visit our website at http://www.the-aps.org/. American Physiological Society, 9650 Rockville Pike, Bethesda MD 20814-3991. ©2012 Int. Union Physiol. Sci./Am. the physiological developments. It is published bimonthly in February, April, June, August, October, and December by (formerly published as News in Physiological Science) publishes brief review articles on m...
متن کاملPatch Clamp Study of Neurotransmission at Single Mammalian CNS Synapses
Single mammalian CNS neurons can be acutely isolated with adherent and functional excitatory and inhibitory synaptic nerve terminals (boutons) using a mechanical dissociation procedure without any enzyme treatment (Vorobjev, 1991; Haage & Johansson, 1998; Rhee et al., 1999). This ‘synaptic bouton’ preparation is particularly suitable for physiological and pharmacological investigations of mamma...
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ژورنال
عنوان ژورنال: The Journal of Physiology
سال: 1987
ISSN: 0022-3751
DOI: 10.1113/jphysiol.1987.sp016750