A Nuclear-Polyhedrosis Virus of Spodoptera frugiperda Isolated in Puerto Rico.

In vitro transactivation of baculovirus early genes by nuclear extracts from Autographa californica nuclear polyhedrosis virus-infected Spodoptera frugiperda cells.

Nuclear extracts, prepared from Autographa californica nuclear polyhedrosis virus-infected Spodoptera frugiperda cells during a time course of infection, were analyzed for activation of early gene transcription and for late gene transcription. The templates used in the in vitro transcription assays contained promoters for baculovirus genes that have been classified as immediate early, delayed e...

Viral Transcription During Autographa californica Nuclear Polyhedrosis Virus Infection: a Novel RNA Polymerase Induced in Infected Spodoptera frugiperda Cells.

Autographa californica nuclear polyhedrosis virus-specific RNA synthesis in isolated nuclei of Spodoptera frugiperda cells in culture was monitored at different times postinfection. Up to 8 h postinfection viral RNA synthesis remained sensitive to 5 mug of alpha-amanitin per ml. During the course of infection this sensitivity decreased, and at 24 h postinfection RNA synthesis was completely res...

Replication of a nuclear polyhedrosis virus in a continuous cell culture of Spodoptera frugiperda: purification, assay of infectivity, and growth characteristics of the virus.

Nonoccluded virus, polyhedra, and occluded virus were purified from a continuous cell culture of Spodopera frugiperda infected with nuclear polyhedrosis virus. The optimal temperature for the replication and lateral transmission of infectivity for the nuclear polyhedrosis viruses (NPV) in cell culture was 27 C. End-point dilution and plaque assay procedures for the measurement of infectivity ar...

Characterization of Cry1F Resistance in Fall Armyworm, Spodoptera frugiperda (J.E. Smith) Obtained from Puerto Rico and Florida

Physical Map of the DNA of a Mamestra brassicae Nuclear Polyhedrosis Virus Variant Isolated from Spodoptera exigua

The D N A of a multiply-enveloped nuclear polyhedrosis virus (MNPV) of the cabbage moth Mamestra brassicae (Mb), isolated from the beet armyworm Spodoptera exigua, was analysed using restriction endonucleases SmaI, BamHI, BgllI and PstI. The size of the viral D N A was calculated to be about 152 kilobase pairs. Mapping of restriction sites on the D N A was accomplished by hybridization of 32 P-...