A novel β-glucanase gene fromBacillus haloduransC-125
نویسندگان
چکیده
منابع مشابه
A novel efficient β-glucanase from a paddy soil microbial metagenome with versatile activities.
BACKGROUND Cellulose, an abundant and renewable polysaccharides, constitutes the largest resource for bioconversion of biofuels. Plant polysaccharides hydrolysis is catalyzed by cellulases, which include endoglucanases, exoglucanases, and β-glucosidases. Converting cellulose and hemicellulose to short chains of oligosaccharides by endo-/exoglucanases is the key step for biofuel transformation. ...
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The endo-β-1,4-glucanase gene celE from the anaerobic fungus Orpinomyces PC-2 was placed under the control of an alcohol oxidase promoter (AOX1) in the plasmid pPIC9K, and integrated into the genome of a methylotrophic yeast P. pastoris GS115 by electroporation. The strain with highest endo-β-1,4-glucanase activity was selected and designed as P. pastoris egE, and cultivated in shaking flasks. ...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 2005
ISSN: 0378-1097
DOI: 10.1016/j.femsle.2005.05.009